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|ELISA Assay Forum ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.|
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ELISA determination of EGFR
I aim to implement a method to determine the expression of EGFR in my fibroblasts using ELISA. I would like to attach the whole lysed protein to the well surface and then incubate with the primary and then secondary attached with Alexa Fluor 488.
I need to know whether I can coat the 96 well plates with some protein I obtained (I used 1 % SDS and phosphorylation inhibitors), what cell lysis and plate coating buffers are the best to use, and a protocol to coat the plates.
Please let me know your protocols and ideas to do things as planned.
|coating , determination , egfr , elisa , lysis buffer|
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