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-   -   Scoring an ELISA (http://www.molecularstation.com/forum/elisa-assay-forum/65685-scoring-elisa.html)

nickcoe 04-15-2009 02:33 PM

Scoring an ELISA
 
I've been detecting the presence of an enzyme in plasma using an adapted ELISA protocol with a positive control from pooled patient samples. The assay works great and I've got some good results however I would like some help in interpreting them and scoring them in effect. I presume that you go off the titre or end point i.e. how many times the sample is diluted before the 'signal' or colour disappears. So I have the end point for the positive control and then end point for an unkown sample but when can I say for instance that a sample is negative or positive for the enzyme? Do you compare the titres of the control and sample directly to produce a ratio or is there a sort of 'standard' titre which you say make the sample positive for your protein??

Any help would be most gratefully received.


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