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Determining an Endpoint Titer in ELISA Assay

Determining an Endpoint Titer in ELISA Assay - ELISA Assay Forum

Determining an Endpoint Titer in ELISA Assay - ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


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Old 01-28-2009, 04:35 AM
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Default Determining an Endpoint Titer in ELISA Assay



Hello
How does one determine an endpoint titer in an ELISA assay?
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Old 01-28-2009, 04:35 AM
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Default Re: Determining an Endpoint Titer in ELISA Assay

Hello there, to determine the endpoint titer for an ELISA (indirect), this is the first time I have been asked to report data as endpoint titers and I am not sure what is meant by this. I typically report data in OD units at a certain Ab dilution.
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Old 02-17-2009, 11:10 PM
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Default Re: Determining an Endpoint Titer in ELISA Assay

You calculate a cutoff (usually 2 times the standard deviation of the mean of the negative control). the OD that is right above that cutoff is the dilution you report as the endpoint titer. For example, if the cutoff is calculated to be OD of 0.200 and there is a reading of 0.250 at dilution 1:250 ... then you report 250 as the reciprocal endpoint titer.
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Old 01-22-2012, 08:11 PM
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Default Re: Determining an Endpoint Titer in ELISA Assay

cutoff value is usually Mean of negative control + 2 times the standard deviation of the mean of the negative control.

for more u can read this paper "Journal of Immunological Methods 2211998.3541"
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