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DNA Binding Protein Assay ELISA Method

DNA Binding Protein Assay ELISA Method - ELISA Assay Forum

DNA Binding Protein Assay ELISA Method - ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


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Old 01-26-2009, 11:16 AM
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Question DNA Binding Protein Assay ELISA Method



I am trying to optimize DNA binding using colorimetric method. I have antibodies for the DNA binding protein. After immobilizing the DNA to microtiter wells and addition of the DNA binding protein the complexes will be measured using TMB substrate. My problem is I am getting very low absorbance values. I am using 400ng 500bp DNA and the protein 10ng to 80 ng. I can see a saturation at 40ng. But the readings are very low. Please suggest

thanks in advance
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Old 01-26-2009, 11:16 AM
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Default Re: DNA Binding Protein Assay ELISA Method

Hi there, would you please tell me how are you coating your DNA to ELISA plate?
If so, give me the reference.

There may be a method you are following is allowing you to capture only a small fraction of DNA to bind to the plate
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assay , binding , dna , elisa , method , protein


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