Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Protein Science > ELISA Assay Forum
Register Search Today's Posts Mark Forums Read

ELISA Assay Forum ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


Primary Antibody Shelf Life?

Primary Antibody Shelf Life? - ELISA Assay Forum

Primary Antibody Shelf Life? - ELISA Forum. Discuss and post questions including optimization, troubleshooting, protocols and theory of ELISA assays using monoclonal, and polyclonal antibodies or peptides.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-04-2008, 03:04 PM
Pipette Filler
Points: 132, Level: 2 Points: 132, Level: 2 Points: 132, Level: 2
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2008
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Primary Antibody Shelf Life?



Hello,

I was wondering if anybody could tell me whats the average shelf life of a primary antibody in PBS when kept in the fridge.

e.g. if I take an aliqout out of the freezer, and add PBS to it to make a 100 fold dilution, and then keep that in the fridge (and make further dilutions from that as needed gradually till it is finished.)

How long will this solution last in the fridge??


Many Thanks
Reply With Quote
  #2  
Old 12-05-2008, 02:55 PM
butters's Avatar
M.D/Ph.D
Points: 4,938, Level: 47 Points: 4,938, Level: 47 Points: 4,938, Level: 47
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2008
Location: Malaysia
Posts: 315
Thanks: 25
Thanked 68 Times in 63 Posts
Default Re: Primary Antibody Shelf Life?

hmmmm i'm not sure i don't take chances. i aliquot in small amount and when needed then thaw and use finish it. i am not sure if adding additive would help to make sure it last longer...

just read somewhere that it is suggested to use BSA at 2% when store in 4C but you would have to check whether ur antibody got degrade or not after that. sodium azide and other additive maybe possible too. if you have molecular manual, it may be best to cross check it... sorry i don't have any manual currently ... who want to donate to me? lol
Reply With Quote
The Following User Says Thank You to butters For This Useful Post:
madvet (12-25-2008)
  #3  
Old 12-18-2010, 01:12 PM
Zagami's Avatar
Ph.D Doctorate
Points: 4,035, Level: 42 Points: 4,035, Level: 42 Points: 4,035, Level: 42
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2009
Location: Tabaka Mission Hospital - Kiisi -Kenya & Italy Sicily
Posts: 159
Thanks: 2
Thanked 28 Times in 24 Posts
Default Re: Primary Antibody Shelf Life?

Stabilized unconjugated antibodies ready-to-use format : suspend between 0.001 and 0.1 mg/ml poly- or monoclonal antibody in stabilizing diluent (dissolve in 700 ml of dH2O, phosphate buffer 0.1 M or Tris-HCl buffer 0.1 M, 8.5 g NaCl, and adjust pH at 7.2. Then dissolve 5 g BSA, 40 mg Congo Red [Direct Red 28] and 0.5 g NaN3. Check pH, adjuste if necessary at 7.2 0.1, and to take 1 liter with dH2O. Mix gently and filter on 0.2 m filter, aliquote in 10 ml glass dark bottle and store at 4 C, stable one year.
Reply With Quote
Reply

Tags
antibody , elisa , life , primary , primary antibody , shelf


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Breakthrough in Cosmology Kazmer Ujvarosy Botany Forum 0 05-21-2004 06:50 AM
Breakthrough in Cosmology Kazmer Ujvarosy Forum Biologie 0 05-21-2004 06:32 AM
Physics and Life - by Paul Davies Laurent Physics Forum 6 01-15-2004 04:17 PM


All times are GMT. The time now is 11:54 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.14052 seconds with 16 queries