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Electrophoretic Mobility Shift Assay Forum EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


Binding buffer

Binding buffer - Electrophoretic Mobility Shift Assay Forum

Binding buffer - EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


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Old 07-29-2013, 08:37 AM
Pipette Filler
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Default Binding buffer



Hi
I am currently trying to get an EMSA using TATA binding protein to work but at the moment I cannot even see a shift using a positive control!

After speaking to a couple of collegues we are thinking it could be down to the binding buffer.

Is there a basic recipie or does it need to be taylor made for different proteins.

The one that I have used consists of, 100mM Tris, 500mM NaCl, 10mM DTT with 25mM DTT/2.5% Tween-20 added seperately into the mixture.

Any help or even a standard type protocol would be great, thanks!
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