i would like to perform an Emsa for a transcription factor to a potential DNA-binding site.
To produce the protein I intend to use the TnT quick coupled transcription/translation kit from Promega using a PCR-generated template.
Is it necessary to label the produced protein (i.e. to use labelled methionine or any other labelling) and detect it using SDS page? Or can I simply perform an unlabelled reaction and use it directly for the EMSA?
I am asking this, because the only protein possible to be produced is my protein of interest from its cDNA.
Thank you very much. Any would be greatly appreciated.