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Electrophoretic Mobility Shift Assay Forum EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


Protein preparation-labelling necessary?

Protein preparation-labelling necessary? - Electrophoretic Mobility Shift Assay Forum

Protein preparation-labelling necessary? - EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


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Old 03-06-2013, 12:19 PM
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Default Protein preparation-labelling necessary?



Hello,

i would like to perform an Emsa for a transcription factor to a potential DNA-binding site.

To produce the protein I intend to use the TnT quick coupled transcription/translation kit from Promega using a PCR-generated template.

Is it necessary to label the produced protein (i.e. to use labelled methionine or any other labelling) and detect it using SDS page? Or can I simply perform an unlabelled reaction and use it directly for the EMSA?

I am asking this, because the only protein possible to be produced is my protein of interest from its cDNA.

Thank you very much. Any would be greatly appreciated.
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