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|Electrophoretic Mobility Shift Assay Forum EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.|
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EMSA: cell extract vs purified protein
I am planning to perform EMSA with a supposedly DNA binding protein and a 22-mer oligo DNA. But I was wondering what's the difference between using purified protein fraction and cell extract extracts + noncompetitve DNA such as dIdC? My protein is transiently transfected to human cells using lipofectamine 2000.
I already can have my protein of interest purified from 293T human cells using anti-FLAG beads, but I can only get this much (see attached picture) protein. It's also time consuming and costly. Is this much protein expression enough for whole cell EMSA?
|cell , emsa , extract , protein , purified|
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