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Electrophoretic Mobility Shift Assay Forum EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


separation of two PCR prodcuts of similar size

separation of two PCR prodcuts of similar size - Electrophoretic Mobility Shift Assay Forum

separation of two PCR prodcuts of similar size - EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


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Old 08-03-2012, 04:32 PM
Pipette Filler
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Default separation of two PCR prodcuts of similar size



Hi,

I need help.

I have done a PCR when there are two products with similar size. They have a size about 1000 pb. I have separated them in a polyacrilamide gel, 8% T and 1,28% C, for 7 hours but aren't well separated from each other. How can I have more resolution of separating of these PCR products?. I need sequencing each.

Thanks
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