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separation of two PCR prodcuts of similar size
I need help.
I have done a PCR when there are two products with similar size. They have a size about 1000 pb. I have separated them in a polyacrilamide gel, 8% T and 1,28% C, for 7 hours but aren't well separated from each other. How can I have more resolution of separating of these PCR products?. I need sequencing each.
|pcr , prodcuts , separation , similar , size|
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