separation of two PCR prodcuts of similar size
 

Hi,

I need help.

I have done a PCR when there are two products with similar size. They have a size about 1000 pb. I have separated them in a polyacrilamide gel, 8% T and 1,28% C, for 7 hours but aren't well separated from each other. How can I have more resolution of separating of these PCR products?. I need sequencing each.

Thanks