Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Electrophoretic Mobility Shift Assay Forum (http://www.molecularstation.com/forum/electrophoretic-mobility-shift-assay-forum/)
-   -   separation of two PCR prodcuts of similar size (http://www.molecularstation.com/forum/electrophoretic-mobility-shift-assay-forum/86170-separation-two-pcr-prodcuts-similar-size.html)

Susan_ 08-03-2012 04:32 PM

separation of two PCR prodcuts of similar size
 
Hi,

I need help.

I have done a PCR when there are two products with similar size. They have a size about 1000 pb. I have separated them in a polyacrilamide gel, 8% T and 1,28% C, for 7 hours but aren't well separated from each other. How can I have more resolution of separating of these PCR products?. I need sequencing each.

Thanks


All times are GMT. The time now is 05:50 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08894 seconds with 11 queries