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Electrophoretic Mobility Shift Assay Forum EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


Antibody Shift Improves Binding

Antibody Shift Improves Binding - Electrophoretic Mobility Shift Assay Forum

Antibody Shift Improves Binding - EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


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Old 06-24-2011, 07:50 PM
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Default Antibody Shift Improves Binding



I've been doing EMSAs on proteins with an N terminal HA tag and I have noticed that when I add anti-HA antibody to the reaction that it not only supershifts the complex but also seems to improve interaction of my protein with the probe.

Has anyone noticed similar effects when doing supershifts? Is there an explanation for this effect?

Cheers!
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