| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Electrophoretic Mobility Shift Assay Forum EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay. |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
11-14-2010, 05:07 PM
| |||||||||||
| |||||||||||
 Seeing a Shift in a control lane Hello, I am doing EMSAs, and I am seeing a shift in a control lane where the cell have not been stimulated (Ie should not expect to see the wanted protiens in the nuclear extract.) the protein also should not be in a high concentration in the cytoplasm (NFKB) The negative control (no nuclear extract added to the binding reaction) works fine (no shift-->no protien bound) does this mean that I am having nonspecific protiens binding to my DNA? Also, this control lane (no stimulation to cells) has a shift with the same size as the experimental lanes... so I don't even know if the experimental lanes are true based on this. do you think its a problem with the binding reaction, or Nuclear extraction methods? Thanks  |
|
| Tags |
| control , lane , shift |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| question on pcr product in control DNA | molbio123 | PCR - Polymerase Chain Reaction Forum | 2 | 05-04-2010 06:47 AM |
| negative control gives band same as positive control | adiza | PCR - Polymerase Chain Reaction Forum | 4 | 04-26-2010 06:03 PM |
| Shift in all 3 lanes! | liquidkodak | Electrophoretic Mobility Shift Assay Forum | 0 | 10-22-2008 01:37 PM |
| Gel Shift Assay-strange Result | p_Sunil2003 | Protocols and Methods Forum | 0 | 08-08-2008 03:57 AM |
| The chemical shift | Mohammed Farooq | Chemistry Forum | 6 | 02-10-2004 10:39 AM |
Linear Mode
