I have in my lab a LightShift EMSA Kit from Pierce designed for DNA binding. I was wondering if I can use RNA instead of DNA with it.
I compared the manual to RNA EMSA one and the only difference seem to be the Binding buffer:
DNA kit: 10X Binding Buffer, 1 ml, 100 mM Tris, 500 mM KCl, 10 mM DTT; pH 7.5
RNA kit: REMSA Binding Buffer (10X), 1mL, 100mM HEPES (7.3), 200mM KCl, 10mM MgCl2, 10mM DTT
Has anybody experience with that?
Do you think I can try to use the DNA buffer, or I should make up the RNA one (I scare RNase doing that)?