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Electrophoretic Mobility Shift Assay Forum EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


IRP-IRE chemilumiescent EMSA

IRP-IRE chemilumiescent EMSA - Electrophoretic Mobility Shift Assay Forum

IRP-IRE chemilumiescent EMSA - EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


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Old 03-04-2010, 08:33 PM
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Default IRP-IRE chemilumiescent EMSA



Hi Every one,

I need to do IRP-IRE (iron regulatory element) EMSA using chemiluminiscent EMSA. I am not sure for the exact binding nucleotide sequence for biotin laelling. Can you please help me in figuring it out is to find out exact 19-21 nucleotides of IRE that can bing to IRP protein.

Any help is highly appreciated.

Thanks
Jaya
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