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Electrophoretic Mobility Shift Assay Forum EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


2-D electrophoresis

2-D electrophoresis - Electrophoretic Mobility Shift Assay Forum

2-D electrophoresis - EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


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Old 01-28-2010, 10:43 AM
Pipette Filler
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Default 2-D electrophoresis



I am working on a homework problem which has:
You tell your student that you need her to run a 2-D gel from the fraction you retrieved in Part A. You know the following:
Size Charge at pH7
70 kD +6
40 kD +6
30 kD +11
20 kD +3


Draw the relative positions of these proteins on 2-D gel below:
pH
4.0 5.0 6.0 7.0 8.0 9.0 10.0
100



KD




25


How do I know how to relate pH charges at 7.0 relative to the Isoelectric point (pI) of this plot?
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