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Electrophoretic Mobility Shift Assay Forum EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


how to modify EMSA(biotin labled oligos)?

how to modify EMSA(biotin labled oligos)? - Electrophoretic Mobility Shift Assay Forum

how to modify EMSA(biotin labled oligos)? - EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


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Old 02-27-2009, 08:50 PM
Pipette Filler
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Default how to modify EMSA(biotin labled oligos)?



Hi, Rescently I did the EMSA USING the biotin labled Oligo. I can not see the shift band. But I can see the positive control which is the kit provided. thanks
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Old 06-09-2010, 06:12 AM
Pipette Filler
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Default Re: how to modify EMSA(biotin labled oligos)?

Make sure to anneal your labeled oligonucleartide first.
The binding condition, binding buffer, proteinNA ratio, cofactor should be optomised.
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