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Electrophoretic Mobility Shift Assay Forum EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


UV cross-linking using fluorescent probes

UV cross-linking using fluorescent probes - Electrophoretic Mobility Shift Assay Forum

UV cross-linking using fluorescent probes - EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay.


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Old 02-21-2009, 12:45 PM
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Default UV cross-linking using fluorescent probes



Hi all,

I am carrying out UV cross-linking assays to characterise a RNA binding protein. Unusually, I have labelled my RNA oligo with a fluorochrome (IRDye800), instead of using the more conventional use of radioprobes.

The technique seems to be working. However, I am finding it hard to optimise my technique (the correct levels of RNA and protein, non-specificity etc).

Has anyone used fluorescently labelled probes for RNA-Protein cross-linking and would be so kind to share their experiences?

Micke
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