Hi. I am just starting EMSAs for the first time. My promoter sequence is 2.5 kb, and I tried to perform EMSA with this sequence. I found that my probe alone without any nuclear extract did not even enter the gel when I used a 6% gel. Does anyone know the maximum size of an oligo that can be used with EMSA. I realize that such a long sequence would bring many nuclear proteins with it and would possibly make the band very fuzzy, but is it possible to use a longer probe with EMSA? If so, what percentage gel and what conditions are favorable for this? Thanks.