does anyone use the biotin labeled DNA to do EMSA?
I have one question for you. How do you get the labeled DNA?
I synthesized them by oligo company. They are 49bp in length and labeled at 3-end, and purified by HPLC. then, I annealed them according Pierce protocol. Before annealing, I measure the concentration of every single strand by NANA DROP.
after diluted, why is there big difference between different fragments at same amount?
Is there someone who can help figure out what is wrong with the procedure?
thank you in advance!