| |||||||
| Register | Blogs | FAQ | Members List | Calendar | Science Groups New! | Arcade | Search | Today's Posts | Mark Forums Read |
| Electrophoretic Mobility Shift Assay Forum EMSA Forum. Discuss the Electrophoretic Mobility Shift Assay protocols or technique and the assessment of DNA and RNA binding proteins here. Also post questions and troubleshooting about the shift assay. |
 |
 |
| | LinkBack | Thread Tools | Display Modes |
03-20-2008, 01:58 PM
| |||||
| |||||
 Emsa Hello; Would anyone help me answer a question about gel shift? I've been doing 32P labelled shifts. I can get very minor (1%) portion of my probes shifted on the small electrophoresis gels (5cmx7cm). However, when I run my EMSA gels on the big gels (20 cm x10cm) I no longer see the shift. Have you encountered this problem? Thanks for your help.        |
| Today
| ||||
| ||||
 Sponsored Links |
|
03-20-2008, 02:52 PM
| |||||
| |||||
 Re: Emsa I suspect your binding is not very good, and the longer time/more electricity it takes to run the longer gels is letting the complex fall apart. Could you try optimizing the EMSA by, for example, reducing the salt concentration, adding cofactors like Zn or Mg, or running a lower percentage gel? |
|
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| RNA EMSA Labeling Method with Biotin | admin | RNA Techniques Forum | 4 | 03-29-2008 01:09 PM |
| EMSA Protocols Technique Information | admin | Electrophoretic Mobility Shift Assay Forum | 0 | 02-06-2008 06:33 AM |
| Lightshift Pierce Kit for DNA EMSA | moleculardude | DNA Techniques | 0 | 02-04-2007 07:39 AM |
| EMSA problem | Balat | Protocols and Methods Forum | 1 | 11-20-2006 07:39 AM |
| EMSA help...please URGENT | sumo1 | Protocols and Methods Forum | 5 | 09-14-2006 09:50 AM |
Linear Mode
