Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Drosophila Forum
Register Search Today's Posts Mark Forums Read

Drosophila Forum Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.


FRT sequences

FRT sequences - Drosophila Forum

FRT sequences - Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-26-2011, 06:56 AM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default FRT sequences



Hello everyone,

I'm trying to design a construct in which my gene is flanked by FRT sites. I've been looking for the sequence of FRTs and I found many different ones and now I don"t know which one to use. What are the differences? I mean, is there any difference in recombination frequency?

In some construct I find 5'-GAAGTTCCTATTCC-3' immediately ustream of the FRT. Is this sequence part necessary?

Thanks a lot!
Reply With Quote
Reply

Tags
frt , sequences


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Select the most related sequences in a multiple sequence alignment LucasVS Bioinformatics 1 05-27-2011 05:43 AM
Alignment software to compare huge number of peptide sequences or cysteine framework LucasVS Bioinformatics 1 05-05-2011 03:21 AM
clustering/distances for unrelated sequences Thomas Isenbarger Protocols and Methods Forum 3 12-11-2004 08:13 PM
New Saccharomyces Sequences 07/16/03 SGD Sequences Yeast Forum 0 07-16-2003 04:12 PM
New Saccharomyces Sequences 06/28/03 SGD Sequences Yeast Forum 0 06-28-2003 06:30 PM


All times are GMT. The time now is 12:48 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11052 seconds with 16 queries