I have been having problems with a particular stock- eyflp; FRT42D armlacZ
-the problem is at the stage of eclosion. The culture looks pretty ok till pupariation, but afterwards, most of these pupae become black and do not eclose, meaning a heavy loss in terms of final yield in flies that I can use for cross. Also I have noticed that the surface of fly food begins to turn black. Some colleagues have described this phenomenon as viral/bacterial infection of some sort. Is there a way to solve this?
Any help in this would be highly appreciated.