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pupal lethality

pupal lethality - Drosophila Forum

pupal lethality - Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.


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Old 06-03-2011, 06:09 AM
Pipette Filler
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Default pupal lethality



I have been having problems with a particular stock- eyflp; FRT42D armlacZ

-the problem is at the stage of eclosion. The culture looks pretty ok till pupariation, but afterwards, most of these pupae become black and do not eclose, meaning a heavy loss in terms of final yield in flies that I can use for cross. Also I have noticed that the surface of fly food begins to turn black. Some colleagues have described this phenomenon as viral/bacterial infection of some sort. Is there a way to solve this?

Any help in this would be highly appreciated.
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Old 06-13-2011, 11:50 AM
Pipette Filler
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Default Re: pupal lethality

If you think you might have a bacterial/fungal infection in the food, you could try adding the following to your fly food (per 1L):
5ml Propionic acid
2.5g Nipagin (Methyl 4-hydroxybenzoate) in dissolved 25ml 100% ethanol (add when food cooled to around 50C)
Do you have necrotic larvae at all stages (I imagine this is what is turning the top of the food black)? If so, it is more likely to be an infection as you suggest.
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