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Imprecise P-element excison - where does the P-element go?

Imprecise P-element excison - where does the P-element go? - Drosophila Forum

Imprecise P-element excison - where does the P-element go? - Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.


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Old 05-26-2011, 03:38 PM
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Default Imprecise P-element excison - where does the P-element go?



I am trying to generate a genomic deletion by imprecise P-element excision but I was wondering, following excision, where does the P-element go?

Does anyone know what happens to a P-element when it has been excised with a transposase?

Can it randomly re-insert itself into the genome? Or does the break that occurs during excision not lend itself to re-insertion (because of presence or lack of overhangs) and the insert is simply degraded?

If you have any thoughts on this I'd love to hear them...
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