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help!!!

help!!! - Drosophila Forum

help!!! - Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.


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Old 03-28-2011, 07:39 PM
Pipette Filler
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Default help!!!



can anyone please help me to unterstand this:

if you want to carry out the p element mediated ends out targeting, you should in one step determine the location of the inserted p element by crossing them to balancer strain.
But why should the p element not be inserted into the same chromosome where also the target gene is located?

i can not understand this
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Old 03-31-2011, 09:13 PM
Pipette Filler
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Default Re: help!!!

My answer would be, that by using a insertion on another chromosome than you want to target you will then screen for a movement, meaning that it have moved to the
chromosome you wanna target, thereby you can probably discard many candidates that for example not have excised properly. Using a insertion on the same chromosome will not do so. The faster you can eliminate false candidates down to a low number the better.
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