Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Drosophila Forum (http://www.molecularstation.com/forum/drosophila-forum/)
-   -   help!!! (http://www.molecularstation.com/forum/drosophila-forum/76737-help.html)

D2011 03-28-2011 07:39 PM

help!!!
 
can anyone please help me to unterstand this:

if you want to carry out the p element mediated ends out targeting, you should in one step determine the location of the inserted p element by crossing them to balancer strain.
But why should the p element not be inserted into the same chromosome where also the target gene is located?

i can not understand this :confused::sad_cry:

Fredrik 03-31-2011 09:13 PM

Re: help!!!
 
My answer would be, that by using a insertion on another chromosome than you want to target you will then screen for a movement, meaning that it have moved to the
chromosome you wanna target, thereby you can probably discard many candidates that for example not have excised properly. Using a insertion on the same chromosome will not do so. The faster you can eliminate false candidates down to a low number the better.


All times are GMT. The time now is 05:04 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08972 seconds with 11 queries