can anyone please help me to unterstand this:
if you want to carry out the p element mediated ends out targeting, you should in one step determine the location of the inserted p element by crossing them to balancer strain.
But why should the p element not be inserted into the same chromosome where also the target gene is located?
i can not understand this :confused::sad_cry:
My answer would be, that by using a insertion on another chromosome than you want to target you will then screen for a movement, meaning that it have moved to the
chromosome you wanna target, thereby you can probably discard many candidates that for example not have excised properly. Using a insertion on the same chromosome will not do so. The faster you can eliminate false candidates down to a low number the better.
|All times are GMT. The time now is 05:04 PM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved