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attP landing site problem

attP landing site problem - Drosophila Forum

attP landing site problem - Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.


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Old 10-26-2010, 09:47 PM
Pipette Filler
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Exclamation attP landing site problem



Dear all,

the question of phiC31 making our life easier has been made doubtful after a summer of injections...

I have obtained transgenic flies (from pUAST) on 3 different landing sites, two on the second and one on the third. However, I was able to drive the UAS only from the landing site on the third...

Has anybody else come across this sort of problem? Are the available landing sites to be trusted? What is the current opinion on potential positional effects?

Much appreciated,

Ema
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attp , landing , phic31 , positional effects , problem , site , transgenesis


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