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Nuclear cytoplasmic fractionation from drosophila heads for western blotting

Nuclear cytoplasmic fractionation from drosophila heads for western blotting - Drosophila Forum

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Old 05-29-2010, 11:22 AM
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Default Nuclear cytoplasmic fractionation from drosophila heads for western blotting



Hi,

I'm new to the drosophila field and are currently struggling to get a good protocol to separate drosophila heads into cytoplasmic and nuclear fractions for analysis by western blotting. The protocol that I got was working on mouse tissue but the separation does not work very good for drosophila heads in my hands.

Anybody out there who has a good protocol (sample homogenization, buffers, centrifugation steps and antibodies used to confirm separation) and is willing to help me on this issue?

Thanks a lot in advance for your help!
Cheers,
Gernot
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blotting , cytoplasmic , drosophila , fractionation , heads , nuclear , nuclear protein , western , western blotting


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