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Nuclear cytoplasmic fractionation from drosophila heads for western blotting
I'm new to the drosophila field and are currently struggling to get a good protocol to separate drosophila heads into cytoplasmic and nuclear fractions for analysis by western blotting. The protocol that I got was working on mouse tissue but the separation does not work very good for drosophila heads in my hands.
Anybody out there who has a good protocol (sample homogenization, buffers, centrifugation steps and antibodies used to confirm separation) and is willing to help me on this issue?
Thanks a lot in advance for your help!
|blotting , cytoplasmic , drosophila , fractionation , heads , nuclear , nuclear protein , western , western blotting|
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