Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Drosophila Forum
Register Search Today's Posts Mark Forums Read

Drosophila Forum Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.

Working with Fat Bodies

Working with Fat Bodies - Drosophila Forum

Working with Fat Bodies - Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.

LinkBack Thread Tools Display Modes
Old 06-13-2008, 02:19 PM
Ivy McDaniel
Posts: n/a
Default Working with Fat Bodies

Hello all,

I am looking for tips for working with fat bodies. I've noticed that
although I am processing everything the same way, I see very variable
results with RNA extractions from fat bodies, in terms of yield especially.
Occasionally I will get preps where I get no RNA. I am confident that the
RNA isolation protocol is fine, and that I don't have RNAse contamination
(except for in the sample itself). I do my dissections on ice, flash freeze
my tubes immediately, and am doing my dissections as rapidly as possible.

Has anyone worked with fat bodies before and had similar problems? I'm
thinking that my problem is probably coming from dissections, either nicking
the gut or being a little too rough with the fat bodies. Got any good tips
for doing quick, clean dissections of fat bodies?

Thank you all,
Ivy McDaniel
Reply With Quote
Old 01-05-2011, 05:52 AM
Pipette Filler
Points: 22, Level: 1 Points: 22, Level: 1 Points: 22, Level: 1
Activity: 0% Activity: 0% Activity: 0%
Join Date: Jan 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Working with Fat Bodies

Ivy McDaniel
Am also tryin to dissect fat body in adult drosophila melanogaster..... i used fat body driver lines from bloomington....... but still not able to get hold of the fat body for rna isolation and use for my expermients.... so may i know what protocolare you using for dissection of fat body....... As for me when i try to dissect all of it floats in the PBS solution.......
Reply With Quote

bodies , fat , working

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
pENTR1A Transformation not working CambridgeBiochemist Molecular Cloning Forum 1 06-18-2012 06:53 AM
Politics And Cannibalism? Introducing The Dourties, Chelsea, Bill, Hillary, Barrack Obama, George Bush, Jr., And All Of Capital Hill! jon_johnfrancisayres@yahoo.com Microbiology Forum 0 10-06-2007 05:59 AM
working for the man lyrics pj harvey Physics Forum 0 06-28-2005 12:23 PM
Why its all wrong. John Schoenfeld Physics Forum 11 07-11-2003 03:25 AM

All times are GMT. The time now is 10:35 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13804 seconds with 16 queries