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gateway vector - pCASPER

gateway vector - pCASPER - Drosophila Forum

gateway vector - pCASPER - Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.


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Old 11-19-2007, 11:47 AM
Anuj Bhatt
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Default gateway vector - pCASPER



Hi,

I'm trying to source a drosophila transformation vector (like pCASPER4,
but) with attB1 and attB2 sites, to use to generate transgenic drosophila
lines.
I need a basic vector to transfer inserts from a pDONR clone.
The plasmid would have the basic elements for transformation, selection of
transgenics, a gateway cassette,
and would have been tested for fly transformation. Is there such a
(unsophisticated) vector available?
I have visited the plasmid larder at flybase and am confused at the
selection on offer, largely through my ignorance of fruit fly-omics (being a
plant biologist).

Any help will be appreciated.

Anuj
--
Anuj Bhatt
Department of Plant Sciences,
University of Oxford,
South Parks Road,
Oxford OX1 3RB
UK
Tel: 00-44-1865-275815
Mobile: 07742880305
Fax: 00-44-1865-275074
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