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mammalian promoters in insect cells

mammalian promoters in insect cells - Drosophila Forum

mammalian promoters in insect cells - Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.


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Old 03-02-2006, 03:08 PM
Fernando
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Default mammalian promoters in insect cells



Dear fly experts,

I normally work with mammalian transformed cell lines (CHO, Cos, HeLa),
now I would like to do some experiments in drosophila cells (SL2,
KC167). Ideally I could transfect these cells with one of the constructs
I use for mammalian cells, but I am concerned if the promoters (SV40 and
CMV) will work in insect cells.
I searched the net on this problem and found no answer to this question.

Does anybody of you have experience with these promoters in the cell-
lines mentioned above or can refer to some info on the net?

Thanks in advance,

Fernando

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