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Help with Lethal Mutations

Help with Lethal Mutations - Drosophila Forum

Help with Lethal Mutations - Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.


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  #1  
Old 05-19-2004, 06:04 PM
Najeeb Siddiqui
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Default Help with Lethal Mutations



Hi,

I am new in working with Drosophila so this might be a naive question to
some. I have received a piggyback (Exelexis) insertion line (balanced by
TM6B, Tb[1] ) that is embryonic lethal for the insertion. I am wondering if
there is an easy way to identify the dead embryos (due to homozygosity of
the balancer) from the other dead embryos (dead due to homozygosity at the
insertion locus)? I will greatly appreciate any help in this matter.
(Can reply at [Only registered users see links. ])

cheers,

Najeeb


---

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Old 05-20-2004, 06:49 PM
Chris Jones
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Default Help with Lethal Mutations

In article <490D0AFAF3D2D3119F6C00508B6FDF1506965A9D@ex.mshri .on.ca>,
[Only registered users see links. ] (Najeeb Siddiqui) wrote:


Put your insertion chromosome over another balancer with an
easily-scored marker, such as GFP.

Chris Jones

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