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Immunostaining polytenes

Immunostaining polytenes - Drosophila Forum

Immunostaining polytenes - Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.


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Old 01-12-2004, 02:15 PM
Jayashree Prasad-Sinha
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Default Immunostaining polytenes



Hello!
Recently I have been facing a peculiar problem with immunostaining polytene
chromosomes. The stain appears grainy - as if the antibodies are minute
grains, instead of the usual bands. Has anyone faced similar difficulty?
How did you overcome it? What could be causing it? I use the standard
protocol of Paro.
Please send any suggestions to my email. I am not a member of bionet.
Thank you,
Jayashree

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