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#1
01-12-2004, 01:15 PM
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 Immunostaining polytenes Hello! Recently I have been facing a peculiar problem with immunostaining polytene chromosomes. The stain appears grainy - as if the antibodies are minute grains, instead of the usual bands. Has anyone faced similar difficulty? How did you overcome it? What could be causing it? I use the standard protocol of Paro. Please send any suggestions to my email. I am not a member of bionet. Thank you, Jayashree ---  |
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| immunostaining , polytenes |
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