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|Drosophila Forum Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis.|
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Recently I have been facing a peculiar problem with immunostaining polytene
chromosomes. The stain appears grainy - as if the antibodies are minute
grains, instead of the usual bands. Has anyone faced similar difficulty?
How did you overcome it? What could be causing it? I use the standard
protocol of Paro.
Please send any suggestions to my email. I am not a member of bionet.
|immunostaining , polytenes|
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