| |||||||
| Register | Blogs | FAQ | Members List | Calendar | Science Groups New! | Arcade | Search | Today's Posts | Mark Forums Read |
| Drosophila Forum Drosophila melanogaster Forum. Discuss and post questions about FlyBase, Drosophila cloning, fruit fly gene crosses expression and analysis. |
 |
 |
| | LinkBack | Thread Tools | Display Modes |
04-13-2008, 09:11 AM
| |||||
| |||||
 DNA Cleanup from Drosophila Hi all, I need to cleanup my DNA constructs to be used for Drosophila fly embryo injections. I found out that I need to phenol-chloroform cleanup the DNA after maxi/mini preps because the DNA columns introduce a substance which interferes with fly embryo development. My inital DNA that I have was maxi-preped and is at around 300ng/ul. With 60/80 of 1.8. so its not that bad... but what do you guys think?        |
| Today
| ||||
| ||||
 Sponsored Links |
|
04-14-2008, 03:20 PM
| |||||
| |||||
| Re: DNA Cleanup from Drosophila The injection company we use asks for midi-maxi prep quality DNA -- we usually send them Qiagen Midiprep kit-prepared DNA and rarely have a problem. That said, a phenol-chloroform extraction can't hurt. |
|
| Tags |
| cleanup, dna, drosophila, fly |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Funny BLAST Results on Drosophila | maymif | Bioinformatics | 1 | 12-17-2007 03:12 PM |
| Specialized hepatocyte-like cells regulate Drosophila lipid metabolism | admin | Science News and Views | 0 | 12-05-2006 07:06 AM |
| Extracting DNA from Drosophila salivary gland squash slides | davidallenjohnson | DNA Techniques | 3 | 11-19-2006 01:16 AM |
| Drosophila genetics software | kmunson779 | Bioinformatics | 2 | 11-01-2006 08:18 PM |
Linear Mode
