Go Back   Molecular Biology Forum > Molecular Research Topics Forum > Molecular Biology Techniques > DNA Techniques
Register Blogs FAQ Members List Calendar Science Groups New! Arcade Search Today's Posts Mark Forums Read

DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


dna Videos
bacterial-dna-ligation-transformation
bacterial-dna-ligation-transformation Bacterial DNA Transformation Video. Learn how to transform ligations into bacteria.


Isolation of DNA

DNA Techniques

Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.



Register Molecular Biology Forums
Reply
 
LinkBack Thread Tools Display Modes
  #1 (permalink)  
Old 02-16-2007, 05:03 PM
Pipette Filler
Points: 845, Level: 16Points: 845, Level: 16Points: 845, Level: 16
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Feb 2007
Posts: 0
Aoife Dunne RSS Feed
Default Isolation of DNA

Hi,

I'm a third year student of Biopharmaceutical & Healthcare Science in Galway-Mayo Institute of Technology, Ireland.

As part of the course, I have been studying the topic of Biotechnology. One of the experiments I have completed is the 'Isolation of DNA from buccal cells'. The protocol stated to use 95% ethanol to precipitate the DNA from solution. However, other protocols I have read specify the use of ice cold ethanol for this particular step in DNA isolation. So, I was wondering could anyone tell me if the ethanol should be ice cold or not?

Also, I would like to know the mechanism by which ethanol precipitates DNA.

Once the DNA is precipitated, I was wondering how long should the extracted DNA take to dissolve in 1 x SSC (15mM sodium citrate, 150mM NaCl, pH 8.0) buffer?

Finally, would someone be able to recommend a source from which a large amount of DNA can be extracted?

Thanks for all the help.
Aoife
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Alt Today
Advertising
Google Adsense
 
This advertising will not be shown
in this way to registered members.
Register your free account today
and become a member on
Molecular Biology Forum
Standard Sponsored Links

Reply

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On
Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Genomic DNA Isolation Protocol moleculardude DNA Techniques 0 07-10-2007 01:01 PM
Isolation of Shuttle Vectors from Yeast and Transformation into E. coli oBWhat Microbiology Forum 0 10-20-2006 03:42 AM
Isolation of caffeine from tea latkan Biochemistry Forum 2 10-10-2006 01:18 PM
caffenine isolation from tea latkan Protocols and Methods Forum 0 10-08-2006 01:59 AM
Plasmid Isolation aftabac Basic Lab Protocols and Techniques 1 08-24-2006 06:17 PM


All times are GMT. The time now is 08:54 PM.


Powered by vBulletin® Version 3.7.1
Copyright ©2000 - 2008, Jelsoft Enterprises Ltd.
Copyright 2005-2007 Molecular Station | All Rights Reserved