| | Re: Help! about transformation E. coli
Welcome to the fourm
well there are many ways to transform E.coli cells,
as there is chemical method mostly by use of CaCl2 treatment, E.coli cells are made competent to transform.
and E.coli cells are also transformed by electroporation.
what method you tried for transformation??
there are many reason for not successful transformation
1 the cells may not be fully competent
2 the size of the vector you use for transformation, as big size vector are not easily transformed
3 the cells might be transformed but your selection procedure may be wrong.....
so there are many explainations are there for transformation failure