I am currently using the FuGene system to transfect HeLa cells with DNA that I have measured using a nanodrop machine.
The nanodrop gave a reading of 400ng/ul of plamid DNA from my extraction.
The FuGene system requires that I do a 3ul Fugene:1ug DNA ratio.
I am a little unsure as to how I should be calculating the volume required of DNA to achieve this ratio.
I know that 1ug=1000 ng/ul, therefore I have divided 400ng/ul by 1000 to give 0.4 ug/ul. Now I am stuck.
This is probably simple but I'm new at this! Thank you.