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Simple DNA Hybridization Buffer

Simple DNA Hybridization Buffer - DNA Techniques

Simple DNA Hybridization Buffer - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 11-02-2012, 09:17 PM
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Unhappy Simple DNA Hybridization Buffer



What is a simple DNA Hybridization Buffer?

I would like to test varying salt conditions to see what is the lowest I can get away for hybridization (yes I know there is a formula and models out there) but we would like to test this. We will be testing on a native poly acrylamide gel.

One paper I read suggested of 25 mM TRIS-HCl with 50 mM NaCl pH 8.3. I tried this (95C for 2 mins and then to 4C) but didn't see any hybridization.

Would SSC be better?

Suggestions?
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