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#1
11-02-2012, 09:17 PM
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 Simple DNA Hybridization Buffer What is a simple DNA Hybridization Buffer? I would like to test varying salt conditions to see what is the lowest I can get away for hybridization (yes I know there is a formula and models out there) but we would like to test this. We will be testing on a native poly acrylamide gel. One paper I read suggested of 25 mM TRIS-HCl with 50 mM NaCl pH 8.3. I tried this (95C for 2 mins and then to 4C) but didn't see any hybridization. Would SSC be better? Suggestions?  |
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| buffer , dna , hybridization , simple |
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