Simple DNA Hybridization Buffer
What is a simple DNA Hybridization Buffer?
I would like to test varying salt conditions to see what is the lowest I can get away for hybridization (yes I know there is a formula and models out there) but we would like to test this. We will be testing on a native poly acrylamide gel.
One paper I read suggested of 25 mM TRIS-HCl with 50 mM NaCl pH 8.3. I tried this (95C for 2 mins and then to 4C) but didn't see any hybridization.
Would SSC be better?
|All times are GMT. The time now is 12:01 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved