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Sequencing length maximum in capillary sequencing

Sequencing length maximum in capillary sequencing - DNA Techniques

Sequencing length maximum in capillary sequencing - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 08-13-2012, 07:38 AM
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Default Sequencing length maximum in capillary sequencing



Hello,

I am trying to sequence a quite long amplicon. I am asking if some of you have experience in sequencing long amplicons. I designed primers for an amplicon that is 1200 bp long. Is that too long?
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Old 09-19-2012, 01:21 PM
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Default Re: Sequencing length maximum in capillary sequencing

1200 bp is not that long, i used to amplify 1400 bp products, it works very well, it just depends on your primer sensitivity,
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