Sequencing length maximum in capillary sequencing
I am trying to sequence a quite long amplicon. I am asking if some of you have experience in sequencing long amplicons. I designed primers for an amplicon that is 1200 bp long. Is that too long?
Re: Sequencing length maximum in capillary sequencing
1200 bp is not that long, i used to amplify 1400 bp products, it works very well, it just depends on your primer sensitivity,
|All times are GMT. The time now is 02:41 PM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved