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-   -   Sequencing length maximum in capillary sequencing (http://www.molecularstation.com/forum/dna-techniques/86206-sequencing-length-maximum-capillary-sequencing.html)

mikefromhell 08-13-2012 07:38 AM

Sequencing length maximum in capillary sequencing
 
Hello,

I am trying to sequence a quite long amplicon. I am asking if some of you have experience in sequencing long amplicons. I designed primers for an amplicon that is 1200 bp long. Is that too long?
:frusty:

namishu95 09-19-2012 01:21 PM

Re: Sequencing length maximum in capillary sequencing
 
1200 bp is not that long, i used to amplify 1400 bp products, it works very well, it just depends on your primer sensitivity,


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