Validating Plasmid DNA

[Artemis2007]

I grew and purified several plasmids for use in our lab. Then I had them sequenced. However, I'm having trouble validating the sequences. I'm using BLAST queries, but not fully understanding and interpreting the results. Also, which precise BLAST search is indicated for this use? Does the plasmid sequence include the primers or backbone?

Any suggestions would be appreciated. Thanks!

[rejusrekanta]

I'm having trouble validating the sequences. I'm using BLAST queries, but not fully understanding and interpreting the results.

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