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Validating Plasmid DNA

Validating Plasmid DNA - DNA Techniques

Validating Plasmid DNA - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 05-04-2012, 10:39 PM
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Default Validating Plasmid DNA



I grew and purified several plasmids for use in our lab. Then I had them sequenced. However, I'm having trouble validating the sequences. I'm using BLAST queries, but not fully understanding and interpreting the results. Also, which precise BLAST search is indicated for this use? Does the plasmid sequence include the primers or backbone?

Any suggestions would be appreciated. Thanks!
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Old 07-24-2012, 05:16 AM
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Default Equipment Validation

I'm having trouble validating the sequences. I'm using BLAST queries, but not fully understanding and interpreting the results.
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Old 08-20-2012, 10:57 AM
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Old 08-22-2012, 05:21 AM
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