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Stability of DNA at 65 C

Stability of DNA at 65 C - DNA Techniques

Stability of DNA at 65 C - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 03-23-2012, 06:00 PM
Pipette Filler
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Default Stability of DNA at 65 C



I left a sample of vector + insert that I was ligating on a heat block (at 65 C) to inactivate the ligase. But, by accident, I left it there for upwards of 40 minutes (at 65 C the entire time). I have removed it and put it at -30 C now, but is my DNA sample okay? Can I still use it, or is my sample ruined?
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