Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > DNA Techniques
Register Search Today's Posts Mark Forums Read

DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.

DNA Endonuclease digestion

DNA Endonuclease digestion - DNA Techniques

DNA Endonuclease digestion - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.

LinkBack Thread Tools Display Modes
Old 03-08-2012, 03:44 PM
Pipette Filler
Points: 4, Level: 1 Points: 4, Level: 1 Points: 4, Level: 1
Activity: 0% Activity: 0% Activity: 0%
Join Date: Mar 2012
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default DNA Endonuclease digestion

I am currently trying to insert a gene of interest from a plasmid into a different empty plasmid. In order to cut the gene of interest from the plasmid, I know I have to use restriction enzymes. However, since the plasmid is circular, would I have to cut the plasmid with two different restriction enzymes or one restriction enzyme that cuts at two places? Also, if the restriction enzyme is supposed to match the enzyme I will be using for the empty plasmid, how is this possible if I use two different restriction enzymes for the plasmid containing my gene of interest?

I'm just confused about the details of this procedure because all of the research I have found online relates to a gene of interest that is not in a plasmid.

If anyone could help me, that would be great. Thanks!
Reply With Quote

digestion , dna , endonuclease

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
BSAI digestion RNAworld PCR - Polymerase Chain Reaction Forum 0 11-04-2011 12:25 AM
Digestion problems Isyy Molecular Cloning Forum 2 02-22-2011 01:08 PM
Scaling-up of partial digestion waikhay Protocols and Methods Forum 1 08-14-2007 02:05 AM
Quesion about DNA gel digestion Jayakumar, R Protocols and Methods Forum 0 07-31-2007 02:26 PM
Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease admin Science News and Views 0 12-05-2006 08:06 AM

All times are GMT. The time now is 08:30 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11080 seconds with 16 queries