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Need help diluting DNA controls

Need help diluting DNA controls - DNA Techniques

Need help diluting DNA controls - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


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Old 10-07-2011, 08:44 PM
Pipette Filler
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Default Need help diluting DNA controls



I need help diluting DNA for sensitivity controls. I have a cell line that is 50% (het) for my mutation of interest. I need to dilute the cell line DNA down to a 10% mutant, 1% and 0.1% mutant.

What volumes would I use of a WT diluent to get to these concentrations. The wild type I am using is blood genomic DNA.

Thanks
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