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Need help diluting DNA controls
I need help diluting DNA for sensitivity controls. I have a cell line that is 50% (het) for my mutation of interest. I need to dilute the cell line DNA down to a 10% mutant, 1% and 0.1% mutant.
What volumes would I use of a WT diluent to get to these concentrations. The wild type I am using is blood genomic DNA.
|controls , diluting , dna|
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