Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > DNA Techniques
Register Search Today's Posts Mark Forums Read

DNA Techniques Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


Mol mixture

Mol mixture - DNA Techniques

Mol mixture - Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 12-29-2006, 02:03 PM
tuttu's Avatar
Pipette Filler
Points: 1,675, Level: 24 Points: 1,675, Level: 24 Points: 1,675, Level: 24
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2006
Posts: 24
Thanks: 0
Thanked 0 Times in 0 Posts
Lightbulb Mol mixture



Hi people,

I have a lil question. I make a mix ture of protein, DNA, RNA, proteinases, DNAses and RNases and incubate them at 30 degrees after half an hour wat will remain the most in the mixture?? I think i know the ans still i wud appreciate replies.

Cya and a Very HAPPY NEW YEAR TO ALL!!

God bless
Reply With Quote
  #2  
Old 12-29-2006, 10:21 PM
molecule2005's Avatar
Ph.D Doctorate
Points: 4,766, Level: 47 Points: 4,766, Level: 47 Points: 4,766, Level: 47
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2005
Posts: 176
Thanks: 0
Thanked 6 Times in 6 Posts
Default Re: Mol mixture

Hello Tuttu!

My feeling (actually I know cause I have done it), is that there will be nothing but the DNAses, the RNases, and the proteases.
Even the proteases eventually will digest the enzymes leaving little much nothing

DNA, RNA and proteins are digested in minutes with even low Units of Enzymes

Happy New Year to you Tuttu!!!

All the best to you and your family!
Reply With Quote
  #3  
Old 01-02-2007, 04:15 AM
kalaig's Avatar
Pipette Filler
Points: 1,657, Level: 24 Points: 1,657, Level: 24 Points: 1,657, Level: 24
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2006
Location: India
Posts: 20
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Mol mixture

The polymers may be converted to monomers Prots--> aa, DNA -->nucleotides, RNA--->nuclecleotides. But unless there are other metabolic enzymes or presence of microbes which can metabolize the monomers, monomers will remain and may interact with each other!
Reply With Quote
  #4  
Old 01-03-2007, 12:10 AM
oBWhat's Avatar
Graduate Student
Points: 3,826, Level: 41 Points: 3,826, Level: 41 Points: 3,826, Level: 41
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2006
Posts: 124
Thanks: 0
Thanked 7 Times in 7 Posts
Default Re: Mol mixture

Very interesting post... makes you think!
Reply With Quote
Reply

Tags
mixture , mol


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
How would you separate a binary mixture of AgCl and PbCl2? ruby Chemistry Forum 0 07-02-2009 10:26 AM
Problem with protein standard mixture Bahara Protein Science 0 07-21-2008 05:12 PM
calculate volume of buffer in reaction mixture spicooli Biochemistry Forum 2 10-22-2007 03:36 AM
Acetonitrile recovery from Sulfuric Acid, Water and Acetonitrile Mixture. venkateswara reddy g Organic Chemistry Forum 0 09-06-2004 02:05 PM
H2/CO2 binary mixture phase equilibria data Jian Peng Chemistry Forum 4 07-22-2003 01:51 PM


All times are GMT. The time now is 08:43 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12651 seconds with 16 queries