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DNA Techniques

Post questions and discuss DNA techniques and protocols such as DNA extraction, PCR, and the study of DNA-binding proteins.



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  #1 (permalink)  
Old 12-29-2006, 01:03 PM
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Lightbulb Mol mixture

Hi people,

I have a lil question. I make a mix ture of protein, DNA, RNA, proteinases, DNAses and RNases and incubate them at 30 degrees after half an hour wat will remain the most in the mixture?? I think i know the ans still i wud appreciate replies.

Cya and a Very HAPPY NEW YEAR TO ALL!!

God bless
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  #2 (permalink)  
Old 12-29-2006, 09:21 PM
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Default Re: Mol mixture

Hello Tuttu!

My feeling (actually I know cause I have done it), is that there will be nothing but the DNAses, the RNases, and the proteases.
Even the proteases eventually will digest the enzymes leaving little much nothing

DNA, RNA and proteins are digested in minutes with even low Units of Enzymes

Happy New Year to you Tuttu!!!

All the best to you and your family!
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Old 01-02-2007, 03:15 AM
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Default Re: Mol mixture

The polymers may be converted to monomers Prots--> aa, DNA -->nucleotides, RNA--->nuclecleotides. But unless there are other metabolic enzymes or presence of microbes which can metabolize the monomers, monomers will remain and may interact with each other!
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Old 01-02-2007, 11:10 PM
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Default Re: Mol mixture

Very interesting post... makes you think!
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