I'm a student working on a project regarding LAMP. Loop-mediated isothermal amplification (LAMP) is a novel technique to amplify nucleic acids under isothermal condition. But so far i haven't been able to produce a positive amplification reaction.
I was wondering if any of you have any experience with LAMP using Bst polymerase to amplify DNA?
I know that primer design is a crucial factor in LAMP, but my primer were made using the default parameters. (primerexplorer.jp/e site is now down prob because of the Japan disaster)
Any feedback is highly appreciated