Originally Posted by msarah
I am also working on a lamp process. Did not get results with 4 primers so moved onto the 6 primer protocol and got excellent results with calcein, HNB and Mn added. Now I keep getting positive results on everything. Have remade the buffers etc with no results. Cannot think of any contamination source. Have you guys had the same problem at any point in your work?
I m a student working on LAMP . Im not getting results even with 6 primers. I designed my primers In primer explorer3 software.
I used PEG and FICOLL, then also i didnt get results.
In all reactions i got only primer dimers.
Kindly help me to get some result. If i get LAMP results i can finish half of my project.