| | Re: (LAMP) Problems with Loop-mediated isothermal amplification
I've read all your post, but still not so clear how you can solve the FALSE-POSITIVE problem.
That is the main issue I concerned about.
I think my primers are good, have used them for years. All my positive come with positive, occasionally I will encounter with a false-positive. In gel, it just has a light band but not ladder-like pattern.
I have totally no idea why they come as false-positive. Because I once prepare all reagents in one tube, then distributed 3/4 of the mixture to 3 others which were negative controls, and only the one I used to prepare give me false-positive.
So I prepared them separately, but can still get one false-positive.
But sometimes, it just runs so good, positive comes to positive, negative comes to positive.
Any idea?... Really appreciated~ Hope to discuss with you guys, hopefully we can all get rid of these issues!