Originally Posted by MCaldera
is someone using HNB? I'm trying to use 3 mM (1 microliter) but i didnt see change in color. does someone can send me the recipe ?? I will appreciate a lot. Also I was having goofd amplification results but now all my samples got positive. Do you have any idea how can I deal with this problem ?
False positive in your reaction are due to cross contamination of your LAMP product.
1. use different sets of pipettor & tips for prepare master mix and post LAMP reaction
2. After the reaction, Do not open the tubes at the same work place where you prepare your master mix
3.Replace all your reagents, decontaminate your pipettor and start fresh your reaction at new work station/ in biosafety cabinet.