Originally Posted by aahu
I found that the PCR is more sensitive than my LAMP assay. Does anybody encounter similar problem? How to increase the sensitivity of the LAMP assay? Thank you.
I can come up with some reasons. The first is your enzyme, it's efficacy may have been decreased beacuase of any reasons including bad storage condition. Secondely, Your primers may be dagraded not totally since you have your reactions performed. Some times this happens in molecular process especially in PCR, at first you see sharp bands,then with the same condition the band in your gel gets pale and pale till it disapears. dNTP mix can also be affected by so many freez thawing. decreasing strigency may help for example by increasing MgSO4. Any how to tackle you problem I suggest you work with new reagents if possible.